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Fitosociologia 41 (1) S1 2004

pag. 193-196: Development of molecular tools for the identification of free nitrogen-fixing bacteria: a contribution to the disclosure of soil microbial biodiversity

L. Aquilanti, I. Mannazzu & F. Clementi

DiSA Università Politecnica delle Marche, Via Brecce Bianche, I-60131 Ancona; e-mail: anmicro@univpm.it

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The preservation of microbial biodiversity offers indisputable benefits, both from an environmental and an economic point of view, due to the essential role of micro-organisms in the environment, and to their huge biotechnological potentiality.
Free nitrogen fixing bacteria (FNFB) represent a group of micro-organisms broadly dispersed in soil, water and sediments, which give a remarkable contribution to nitrogen fixation in the biosphere and could be usefully exploited as biological fertilisers and for other biotechnological applications. With the aim of providing a new, powerful tool for the study of these micro-organisms isolated from soil, we developed a method based on the 16S rDNA Amplified Ribosomal DNA Restriction Analysis (ARDRA) which was successfully used in order to identify 59 isolates from soils of central Italy.


ARDRA, Azotobacteraceae, Biodiversity, Free nitrogen fixing bacteria, Nitrogen fixation.